一株H_5N_1亚型禽流感病毒株NA基因的克隆与序列分析     

陈绍红  刘艳芬  张静  刘铀 《广东海洋大学学报》2007,27(4):85-88

用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。  相似文献   

Dispersion measure variations and their effect on precision pulsar timing     

X. P. You  G. Hobbs  W. A. Coles  R. N. Manchester  R. Edwards  M. Bailes  J. Sarkissian  J. P. W. Verbiest  W. van Straten  A. Hotan  S. Ord  F. Jenet  N. D. R. Bhat  A. Teoh 《Monthly notices of the Royal Astronomical Society》2007,378(2):493-506

We present an analysis of the variations seen in the dispersion measures (DMs) of 20-ms pulsars observed as part of the Parkes Pulsar Timing Array project. We carry out a statistically rigorous structure function analysis for each pulsar and show that the variations seen for most pulsars are consistent with those expected for an interstellar medium characterized by a Kolmogorov turbulence spectrum. The structure functions for PSRs J1045−4509 and J1909−3744 provide the first clear evidence for a large inner scale, possibly due to ion–neutral damping. We also show the effect of the solar wind on the DMs and show that the simple models presently implemented into pulsar timing packages cannot reliably correct for this effect. For the first time we clearly show how DM variations affect pulsar timing residuals and how they can be corrected in order to obtain the highest possible timing precision. Even with our presently limited data span, the residuals (and all parameters derived from the timing) for six of our pulsars have been significantly improved by correcting for the DM variations.  相似文献   

Interaction of linear waves with an array of infinitely long horizontal circular cylinders in a two-layer fluid of infinite depth     

B. Feng  Y.H. Zheng  Y.G. You  B.J. Wu  S.W. Sheng   《Ocean Engineering》2008,35(10):963-982

The linear water wave scattering and radiation by an array of infinitely long horizontal circular cylinders in a two-layer fluid of infinite depth is investigated by use of the multipole expansion method. The diffracted and radiated potentials are expressed as a linear combination of infinite multipoles placed at the centre of each cylinder with unknown coefficients to be determined by the cylinder boundary conditions. Analytical expressions for wave forces, hydrodynamic coefficients, reflection and transmission coefficients and energies are derived. Comparisons are made between the present analytical results and those obtained by the boundary element method, and some examples are presented to illustrate the hydrodynamic behavior of multiple horizontal circular cylinders in a two-layer fluid. It is found that for two submerged circular cylinders the influence of the fluid density ratio on internal-mode wave forces is more appreciable than surface-mode wave forces, and the periodic oscillations of hydrodynamic results occur with the increase of the distance between two cylinders; for four submerged circular cylinders the influence of adding two cylinders on the wave forces of the former cylinders is small in low and high wave frequencies, but the influence is appreciable in intermediate wave frequencies.  相似文献   

2018年1月江苏3次致灾暴雪成因对比分析          下载免费PDF全文

王喜  王琴  向阳  郑炜  李由 《山东气象》2020,40(1):134-143

应用常规天气图资料、FY-2E 云顶亮温(TBB)资料、多普勒雷达观测资料和NCEP/NCAR 1°×1°再分析资料,对江苏2018年1月3—4日(简称“01·04”过程)、1月24—25日(简称“01·25”过程)和1月27—28日(简称“01·27”过程)3次暴雪过程进行了对比分析。结果表明：1)3次暴雪过程都是在500 hPa高空槽、中低层切变线、700 hPa西南急流和地面冷空气的共同影响下产生的;暴雪过程中水汽主要来源于中层,降雪期间逆温层结始终存在。2)不同之处是,“01·04”过程中层暖湿气流先形成,水汽条件更好,而后弱冷空气自低层楔入,促使暖湿气流抬升,上升运动发展更为旺盛;“01·25”过程和“01·27”过程低层先形成冷垫,而后中层暖湿气流增强沿冷垫爬升,冷垫更冷,“01·25”过程逆温更强。3)暴雪过程中TBB稳定低值期基本可以反映强降雪时段;“01·04”过程中有弱对流发展,造成降雪强度大。  相似文献   

“中国天然氧吧”品牌效益评估指标体系构建与应用——以江西省乐安县为例          下载免费PDF全文

谢佳杏  游霞  王嘉琦  李屹东 《气象与减灾研究》2023,46(1):74-79

开展“中国天然氧吧”品牌效益评估,对推动生态产品价值转换具有重要意义。运用层次分析法从生态效益、经济效益、社会效益三个方面构建了“中国天然氧吧”品牌效益评估指标体系,并细化为23个具体指标。结合模糊综合评价法,以江西省乐安县为例进行实际应用检验。结果表明：1）此效益评估体系和评估方法具有科学性和合理性,评价结果可以为政府部门合理开发旅游气候资源、推动“中国天然氧吧”品牌可持续发展提供借鉴。2）乐安县“中国天然氧吧”品牌综合效益属“良好”等级。3）针对低评分指标,提出当地政府需要加强生态环境保护规划、生态旅游开发力度和品牌宣传推广等建议。  相似文献   

室内三维点云空间自动划分与规则化方法北大核心CSCD     

王敬淳  汤圣君  王伟玺  李晓明  李游  谢林甫  朱家松 《测绘通报》2022,(8):93-97

精准空间划分是实现室内语义建模与拓扑结构重建的重要基础。三维点云作为常用的室内空间数据载体,如何基于三维点云进行室内空间语义信息提取与规则化具有重要意义。本文提出了一种基于形态学分割方法实现室内场景的分割,并结合矢量规则化方法完成分割场景的规则化。首先,基于区域增长算法与线性拟合方法提取空间分割要素,通过平面投影生成二进制影像,进而利用距离变换和分水岭算法完成空间分割;然后,对空间分割要素进行线性拟合,进行室内空间格网划分,采用矢栅叠加方法实现空间要素规则化;最后,通过4组实际场景(包含3组ISPRS数据集及1组实际场景采集数据)进行数据验证。试验结果显示,本文提出的室内空间分割与规则化方法可以准确快速地完成室内空间要素的提取。  相似文献   

Emergency alternative evaluation and selection based on ANP,DEMATEL, and TL-TOPSIS     

Ju  Yanbing  Wang  Aihua  You  Tianhui 《Natural Hazards》2014,75(2):347-379

With the increasing occurrence frequency of emergency events, emergency management (EM) has been a very important issue in management science. One of the major activities of EM is to evaluate and select the most desirable emergency alternative(s). This paper proposes a new framework combining the analytic network process (ANP) method, the decision-making trial and evaluation laboratory (DEMATEL) technique, and 2-tuple linguistic technique for order preference by similarity to an ideal solution (TL-TOPSIS) method to solve the emergency alternative evaluation and selection problem. This study has been done in three stages. In the first stage, we use DEMATEL technique to obtain the network relation map (NRM) among emergency alternative evaluation criteria or sub-criteria. In the second stage, we use ANP method to calculate the global weight of each sub-criterion based on the NRM among emergency alternative evaluation sub-criteria. In the third stage, the ratings of emergency alternative with respect to each sub-criterion are described by linguistic items, and the TL-TOPSIS method is used to rank the emergency alternative. Finally, a practical example of urban fire emergency alternative selection is given to illustrate the application of the proposed framework.

  相似文献   

深部开采圆形巷道围岩破损区与支护压力的确定     

李铀  袁亮  刘冠学  金学玉  薛俊华  余国锋  彭意  张明 《岩土力学》2014,35(1):226-231

应用经典塑性力学理论确定的圆形巷道围岩弹塑性应力场存在一些问题,这影响深部圆形巷道的稳定性评价及支护设计的合理性。应用塑性力学求解新体系确定的圆形巷道围岩弹塑性应力场准确解,进行了各向同性均质地层中圆形巷道破损区、地层残余强度与支护压力关系方面的研究,取得了如下成果:(1)破损区随开采深度的增加而增大,它们基本呈线性关系。(2)建立了支护压力与巷道埋深、围岩强度、围岩破坏后残余强度、围岩泊松比和重度之间的关系。研究成果可为支护设计提供重要参考。  相似文献   

Quantification of electrical resistance to estimate NaCl behavior in a column under controlled conditions     

S. A. Junejo  Qi You Zhou  M. A. Talpur  Dongqing Wang  Jian-long Yang 《Geochemistry International》2014,52(9):794-804

Accurate prediction of solute transport processes in surface water and its underlying bed is an important task not only for proper management of the surface water but also for pollution control in these water bodies. Key issue in this task is an estimation of parameters as diffusion coefficient and velocity for solute transport both in water body and in the underlying bed. This estimation would greatly help us to understand the deposition and release mechanism of solute across the water-bed interface. In this study, a column experiment was conducted in laboratory to estimate the velocity and diffusion coefficient of sodium chloride (NaCl) in water body and underlying sand layer (bed). The column used with a diameter of 30 cm and a height of 100 cm, was filled with sand at the lower half part and water at the upper half part. Total 64 stainless steel electrodes were installed on its surface around. The sodium chloride solution was injected from the top of the column, and electrical resistance between electrodes was monitored for 71 h. Then the dimensionless resistance breakthrough curve was fitted with one dimensional analytic solution for solute transport and the related diffusion coefficient and velocity parameters were estimated. The results show that the NaCl transport velocity was high in the water body but extremely low in the underlying sand layer (bed). The diffusion coefficient estimated in sand layer coincides with those reported well. This indicates that the electrical resistance based solute transport parameter estimation method is not only effective but also has an advantage of multipoints monitoring. This is useful both in mapping solute transport parameter for solute transport process analysis and in providing parameter input for solute transport numerical modeling.  相似文献   

Site selection in municipal solid waste management with extended VIKOR method under fuzzy environment   总被引：2，自引：2，他引：0  

Hu-Chen Liu  Jian-Xin You  Yi-Zeng Chen  Xiao-Jun Fan 《Environmental Earth Sciences》2014,72(10):4179-4189

Nowadays, selection of the suitable disposal site in municipal solid waste (MSW) management has become a challenge task for the municipal authorities, especially in fast-growing areas. Site selection can be viewed as a complicated multi-criteria decision-making (MCDM) problem requiring consideration of multiple alternative solutions and conflicting quantitative and qualitative criteria. In this paper, linguistic variables, which can be expressed as trapezoidal fuzzy numbers, are used to assess the ratings and weights for the selection criteria. The ordered weighted averaging operator is utilized to transform the fuzzy decision matrix into crisp values considering the decision maker’s attitudinal character. For selecting the best site, the extended VIsekriterijumska optimizacija i KOmpromisno Resenje (VIKOR) method is applied to determine the priority ranking of alternatives. As a result, a hierarchy MCDM model based on fuzzy set theory and VIKOR method is proposed to deal with the site selection problems in the MSW management system. An empirical study in Shanghai, China, is provided and comparison with the existing approach is conducted to illustrate the applicability and benefits of the proposed method.  相似文献